July 25, 2024 (Day 1 – Thursday) |
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| 08:45 – 09:00 | Opening remarks by Johannes Walter | FAU Erlangen-Nürnberg & Alexander Bepperling | Sandoz, Germany | |
| Session 1: New methods and methods development – Part 1 Chair: Johannes Walter | FAU Erlangen-Nürnberg, Germany |
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| 09:00 – 09:25 | Lutz Ehrhardt | Beckman Coulter Life Sciences, Germany | The involvement of Beckman Coulter Life Sciences in analytical ultracentrifugation from 1947 and beyond – A personal perspective |
| 09:25 – 09:50 | Borries Demeler | University of Lethbridge, Canada | What you can learn from multiwavelength AUC |
| 09:50 – 10:15 | Kiichi Hirohata | Osaka University, Japan | Establishment of equilibrium density gradient analytical ultracentrifugation for the analysis of AAV |
| 10:15 – 10:35 | Lukas Dobler | University Konstanz, Germany | Band forming experiments in the AUC |
| 10:35 – 10:55 | Coffee break | |
| Session 2: New methods and methods development – Part 2 Chair: Borries Demeler | University of Lethbridge, Canada |
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| 10:55 – 11:20 | Chad Aaron Brautigam | UT Southwestern Medical Center, USA | Easy, interactive simulations of sedimentation velocity data using SViMULATE |
| 11:20 – 11:45 | Stefan Kuechler | LUM GmbH, Germany | Multidimensional particle and liquid dispersion characterization by advanced analytical centrifugal sedimentation |
| 11:45 – 12:10 | Alexey Savelyev | University of Montana, USA | Ultrascan GMP novel features |
| 12:10 – 12:30 | Moritz Moß | FAU Erlangen-Nürnberg, Germany | Deep learning based synthesis of sedimentation boundaries in centrifugation experiments |
| 12:30 – 14:00 | Lunch | |
| Session 3: Multiwavelength analytical ultracentrifugation – New methodologies and applications Chair: Susumu Uchiyama | Osaka University, Japan |
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| 14:00 – 14:25 | Johannes Walter | FAU Erlangen-Nürnberg & K. David Wegner | BAM, Germany | Size-resolved emission properties of core/shell CdSe/CdS quantum dots via multiwavelength emission analytical ultracentrifugation |
| 14:25 – 14:45 | Saeed Mortezazadeh | University of Lethbridge, Canada | New functions in UltraScan for the processing of multi-wavelength AUC SV and ABDE experiments |
| 14:45 – 15:10 | Paola Ivonne Cardenas Lopez | FAU Erlangen-Nürnberg, Germany | Characterization of polydopamine shells on polystyrene particles by analytical buoyant density equilibrium experiments |
| 15:10 – 15:30 | Coffee break | |
| Session 4: Multi-wavelength analytical ultracentrifugation in biology Chair: Ute Curth | Hannover Medical School, Germany |
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| 15:30 – 15:55 | Susumu Uchiyama | Osaka University, Japan | Analytical ultracentrifugation for the comprehensive characterizations of adeno-associated virus vector |
| 15:55 – 16:15 | Sophia Bird | University of Lethbridge, Canada & University of Montana, USA | Biophysical analysis of PlzA-nucleotide interactions with multi-wavelength analytical ultracentrifugation |
| 16:15 – 16:45 | Aysha Kinjo Demeler | University of Montana, USA | Biophysical characterization of GFP-nanobody complex formation |
| 16:45 – 17:45 | Technical Workshop by LUM GmbH | |
| 18:00 – 19:30 | Dinner | |
| 19:30 – 22:00 | Mixers, trade fair and poster session | |
July 26, 2024 (Day 2 – Friday) |
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| Session 5: Biopharma applications Chair: Alexander Bepperling | Sandoz, Germany |
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| 08:45 – 09:10 | Alexander E. Yarawsky | Bioanalysis LLC, USA | Analytical ultracentrifugation: A platform technology for Proteolysis Targeting Chimeras (PROTACs) |
| 09:10 – 09:35 | Eric S. Day | Genentech, USA | Ionic strength and pH dependence of antibody reversible self-association probed by SV-AUC |
| 09:35 – 10:00 | Ester Serrano | University of Glasgow, UK | A new twist on drug design: modifying metabolism to inhibit virulence |
| 10:00 – 10:20 | Reece Martin | University of Lethbridge, Canada | Development of molecular standards to validate analytical ultracentrifugation instruments for GMP qualification |
| 10:20 – 10:45 | Klaus Richter | Coriolis Pharma, Germany | SV-AUC during the development of LNPs and ATMPs |
| 10:45 – 11:05 | Coffee break | |
| Session 6: Protein applications – Biological interactions Chair: Olwyn Byron | University of Glasgow, UK |
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| 11:05 – 11:30 | Renwick Dobson | University of Canterbury, New Zealand | TRAPped in an elevator: amino-sugar uptake and utilisation by bacteria. |
| 11:30 – 11:50 | Ute Curth | Hannover Medical School, Germany | The impact of von Willebrand factor on immune complexes in hemophilia A |
| 11:50 – 12:10 | Caroline Mas | Université Grenoble Alpes, France | Characterization of Rabies virus phosphoprotein thermoresponsive liquid-liquid phase separation |
| 12:10 – 12:30 | Xiaodong Ye | University of Science & Technology of China, China | Investigation of the complexation between glycinin and block copolymers through analytical ultracentrifugation |
| 12:30 – 14:00 | Lunch | |
| Session 7: Nanoparticles and polymers – Part 1 Chair: Jeffrey Fagan | NIST, USA |
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| 14:00 – 14:25 | Amy Henrickson | Beckman Coulter Life Sciences, Canada | Leveraging analytical ultracentrifugation for comprehensive characterization and quantification of lipid nanoparticles |
| 14:25 – 14:50 | Stephen E. Harding | University of Nottingham, UK | Hydrodynamic tools for assessing the molecular integrity of archaeological consolidants. The Saving Oseberg project |
| 14:50 – 15:10 | Quy Ong | Ecole polytechnique Federale de Lausanne, Switzerland | Characterization of gold nanoparticles by cryogenic electron tomography and analytical ultracentrifugation |
| 15:10 – 15:35 | Ute Resch-Genger | BAM, Germany | Quantifying the number of total and accessible functional groups on nanomaterials |
| 15:35 – 16:00 | Coffee break | |
| Session 8: Protein applications – Protein hydrodynamics Chair: Christine Ebel | Université Grenoble Alpes, France |
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| 16:00 – 16:25 | Patrick Fleming | Johns Hopkins University, USA | The molecular basis for hydrodynamic properties of PEGylated proteins |
| 16:25 – 16:50 | John Correia | University of Mississippi Medical Center, USA | Hydrodynamic and thermodynamic analysis of PEGylated human serum albumin |
| 16:50 – 17:15 | Stephen E. Harding | University of Nottingham, UK | Hydrodynamic comparison of the structure and interactions of the last line of defence antibiotics vancomycin and teicoplanin |
| 17:15 – 17:35 | Wenqi Li | Tsinghua University, China | Poly acidic amino acids sequence drives variation of protein in hydrodynamic properties |
| 18:00 – 19:30 | Dinner | |
| 19:30 – 21:00 | Poster session and get-together | |
July 27, 2024 (Day 3 – Saturday) |
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| Session 9: Nanoparticles and polymers – Part 2 Chair: Amy Henrickson | Beckman Coulter Life Sciences, Canada |
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| 08:45 – 09:10 | Ilya Anufriev | Friedrich Schiller University Jena, Germany | Molecular hydrodynamic characterization of PEG-lipid conjugates |
| 09:10 – 09:30 | Mengdi Chen | East China Normal University, China | Fractionation of colloidal particles: From analytical ultracentrifugation to preparative ultracentrifugation |
| 09:30 – 09:55 | Tianbo Liu | University of Akron, USA | Using analytical ultracentrifugation to explore macroionic solutions by determining critical parameters |
| Session 10: Beadmodeling and hydrodynamic simulations Chair: Karen Fleming | Johns Hopkins University, USA |
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| 09:55 – 10:20 | José Garcia de la Torre | University of Murcia, Spain | Some recent modelling/computation advances in hydrodynamics, analytical ultracentrifugation and scattering techniques |
| 10:20 – 10:45 | Mattia Rocco | IRCCS Ospedale Policlinico San Martino, Italy | Atomic level hydration and bead models hydrodynamics: Parallel GRPY for all and an adjusted ZENO method in US-SOMO |
| 10:45 – 11:00 | Coffee break | |
| Session 11: Cross-disciplinary research Chair: Klaus Richter | Coriolis Pharma, Germany |
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| 11:00 – 11:25 | Emre Brookes | University of Montana, USA | Advances in the US-SOMO Small-Angle Scattering module: UV-Vis spectral data processing, Multi-Angle Light Scattering (MALS) coupled to SAXS data analysis, and improved SEC Gaussian decomposition of not resolved species |
| 11:25 – 11:50 | Ivo Nischang | Friedrich Schiller University Jena, Germany | Molecular hydrodynamic characterization of synthetic polymers for life science and energy applications |
| 11:50 – 12:10 | Lisa M. S. Stiegler | FAU Erlangen-Nürnberg, Germany | Insights into the stability and agglomeration of metal oxide nanoparticles by analytical (ultra)centrifugation |
| 12:10 – 12:30 | Oliver Keatinge Clay | Universidad del Rosario, Colombia | The tail of DNA: Polymers in a density gradient in the AUC still tell new genome stories |
| 12:30 – 12:45 | Closing ceremony | |
| 12:45 – 14:00 | Lunch | |
| 14:00 – 14:30 | Business Meeting | |
| 15:00 – 17:00 | Social event | |
| 18:00 – 19:30 | Dinner for remaining attendees | |